Complex development of biological products for medicine, veterinary and cosmetology

• Complex development of biological products for medicine, veterinary and cosmetology

  The company "Pharma gene" provides services and performs research and development in the following areas:

  1. Patent Research

  2. Gene and protein engineering to create a biopharmaceutical drugs.

  3. The development of non-viral delivery methods of gene therapy constructs.

  4. Development of protein preparations for medicine, veterinary medicine and cosmetology.

  5. Development of stable liquid dosage forms of the recombinant proteins.
  • Development dosage form supporting recombinant proteins for a long time in dissolved form
  • Analysis of the refolding and storage efficiency by the original method

  6. Create drugs based on humanized chimeric monoclonal antibodies.
  • sequencing of the genes encoding the variable domains of a mouse MAb
  • Creating bicistronic vectors containing genes encoding the variable domains of the mouse MAb and the constant domains of human IgG1
  • Preparation of stable CHO cell lines
  • Annotate the variable domains of mouse MAbs using a modified algorithm Kabat - Chothia and molecular modeling conformational homology of the antibody with taking into account the CDR and FR structure, as well as data on VH / VL folding corners in the donor and acceptor antibodies
  • transfer of the CDR from donor mouse monoclonal antibody to the chosen human acceptor antibody
  • Increasing the affinity of humanized antibodies using a combinatorial directed mutagenesis of the predicted amino acid residues that affect the antibody affinity

  7. Development of medical and veterinary preparations for gene therapy
  • Create a vector for gene therapy based on plasmid DNA
  • Development of technology for scalable ion-exchange chromatographic purification of plasmid DNA.